PROTEIN
Apurinic apyrimidinic endonuclease 1/Redox factor-1 (APE1/Ref-1) is a multifunctional protein; its N-terminal region is involved in redox activity and regulates multiple transcription factors, and its C-terminus is involved in base excision DNA repair activity. APE1/Ref-1 is mainly localized in the nucleus and shows dynamic shuttling between the nucleus and cytoplasm in response to various stress stimuli. Recently, it have a reported the possibility for the extracellular secretion of APE1/Ref-1. Elevated level of APE1/Ref-1 were observed in the blood of endotoxemic rats, and in bladder cancer.
| Protein Gene name | APEX1 |
|---|---|
| Other name | APE1, Ref-1, APE1/Ref-1 |
| NCBI Reference Sequence | NM_001641.3 |
| Cat.No | MR-RPAPE-*** |
| AA Sequence | MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRDPNSMPKRGKKGAVAEDGDELRTEPEAKKSKTAAKKNDKEAAGEGPLYED PPDQKTSPSGKPATLKICSWNVDGLRAWIKKKGLDWVKEEAPDILCLQETKCSENKLPAELQELPGLSHQYWSAPSDKEGYSGVGLLS RQCPLKVSYGIGDEEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNP KGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTPYAYTFWTYMMNARSKNVGWRLDYFLLSHSLLPALCDSKIRSKALGSDH CPITLYLAL |
| Size | 0.05, 0.1, 0.5, 1 mg/ml |
| Host | E. Coli |
| Form/ storage solution | Solution, containing 10mM Tris-HClpH8, 50mM Nacl, 1mM DTT, 0.05mM EDTA, 200 µg/ml BSA, 50% glycerol |
| Concentration | 1mg/ml |
| Protein Form | Full Length, Recombinant |
| Protein Tag or Fusion | N-terminal His tag |
| Protein size | 39 kDa |
| Purity | ≥ 95% by SDS-PAGE |
| Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles |
| Product Name | Cat. No | Size | Price (VAT excluded) |
|---|---|---|---|
| APE1/Ref-1 protein, Human, Recombinant (His Tag) | MR-RPAPE-005 | 0.05mg | ￦300,000 |
| APE1/Ref-1 protein, Human, Recombinant (His Tag) | MR-RPAPE-010 | 0.1mg | ￦510,000 |
| APE1/Ref-1 protein, Human, Recombinant (His Tag) | MR-RPAPE-050 | 0.5mg | ￦2,250,000 |
| APE1/Ref-1 protein, Human, Recombinant (His Tag) | MR-RPAPE-100 | 1mg | ￦3,900,000 |
- References
- Robson, C.N. and Hickson, D.I. (1991) Nucleic Acids Res. 19, 5519–5523.
- Demple, B. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 11450–11454.
- Park, M. et al, (2016) Sci Reports 6:23015.